Nucleofection of Insect Cells
For many research protein applications insect cells provide the ideal expression system, as they combine the ability to produce proteins containing eukaryotic post-translational modifications with both high productivity and simple cultivation needs.
Nucleofection provides outstanding transfection efficiencies for most common insect cell lines incl. Sf9 and S2 cells. This facilitates significant time-savings in generation of recombinant baculotiters for baculovirus-mediated protein expression as well as excellent protein yields from transient protein production.
- Up to 82% transfection efficiency and 79% cell viability
- Transient protein expression with high yields
- Time-savings in generation of baculovirus due to excellent efficiency of bacmid delivery
96-well nucleofection format available for fast and standardized screening of bacmid or other vector constructs - Easy-to-follow protocols with cell culture details
Excellent protein production rates of S2 insect cells 24 hours post nucleofection. S2 cells (ATCC) were transfected with insect expression vector encoding the LacZ/β-gal gene. Each bar represents the average β-gal enzyme activity from six replicates 24 h post-transfection, demonstrating the excellent protein production rates achieved by nucleofection already after 24 h. β-gal enzyme activities were at similar levels 48 h after transfection (data not shown).
For more information on S2 and Sf9 nucleofection click on the following:
