Applications of the Nucleofector Technology

The Nucleofector technology is well suited to address specific questions in particular  research areas, but is also an ideal platform for applications (such as RNAi-mediated gene silencing and stable transfections) that transcend such divisions.

 RNAi is a powerful tool for gene silencing in a highly specific and efficient manner. Efficient down-regulation of gene expression by nucleofection of siRNAs has already been published for many cell types (Link Citations List). Using the Nucleofector technology for RNAi is a simple process as the optimal conditions for transfection of siRNA are identical to those for DNA (i.e. as given in our Optimized Protocol for your cell type of choice).

Nucleofection has also been successfully tested for  protein production. Protein amounts up to 5 mg can be produced transiently within just a few days. The Nucleofector technology represents the ideal tool for gene transfer even into difficult-to-transfect cell lines like suspension CHO cells.

Nucleofection is not only very efficient for transient transfection, it can also be used to generate  stably transfected clones especially from difficult-to-transfect cell lines. Such transfectants open new research possibilities including the functional analysis of clones generated from single-integration events and protein production in stably transfected cell lines.

You can also contact our  Scientific Support Team to learn more about recent advances in Nucleofector applications.