Accelerate your Research with the Nucleofector 96-well Shuttle System
- Target validation in primary cells
- High throughput generation of stable clones
- Protein production in suspension cell lines
Based on the well-established Nucleofector Technology amaxa has developed a system for the highly efficient transfection of primary cells and difficult-to-transfect cell lines in 96-well format.
The Nucleofector 96-well Shuttle System consists of three components, the 96-well Shuttle, the Nucleofector II Device and a laptop. Driven by the Nucleofector II, the 96-well Shuttle can deliver DNA directly into the nucleus, allowing even non-dividing primary cells (such as resting T cells or neurons) to be efficiently transfected. siRNA delivery into the cytoplasm, on the other hand, also occurs with up to 99% efficiency.
Optimal Nucleofection conditions depend upon the individual cell type, not on the substrate being transfected. This means that identical conditions are used for the Nucleofection of DNA, siRNA or shRNA vectors. So it is a simple matter to switch back and forth between substrates or to perform co-transfections with DNA and RNA together.
Benefit from
High Efficiencies
- Up to 95% transfection efficiency with DNA combined with viabilities > 95%
- Up to 99% siRNA duplex transfer even in suspension cells
High reproducibility
- Small intra- and inter-plate standard deviations
- No edge effects and no lot-to-lot variance
Access to difficult-to-transfect cell types
- Neurons, T cells and other difficult-to-transfect cell types can be transfected
Ease of use
- Optimized 96-well Nucleofector Kits and protocols for many primary cells and cell lines
Substrate flexibility
- Identical transfection conditions for DNA, siRNA, shRNA, miRNA ...
Transfection flexibility
- Modular 2 x 8 Nucleocuvette plate for scalable throughput
- Up to 96 independent programs can be run per plate
- Variable cell numbers - from 104 - 106 cells per reaction
Rapid optimization
- Optimization of any difficult-to-transfect cell line in just one plate
Safety
- Disposable plates minimize the risk of cross contamination
- Innovative conductive polymer - no metal ion release
HTS compatibility
- Automatic processing of a 96-well plate in 3-4 min
- Fulfills prerequisites for Liquid Handling integration
